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Pel-Freez primary antibodies against th
Primary Antibodies Against Th, supplied by Pel-Freez, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+antibody+against+th/anti+rabbit+th/pm41771413-126-0-4
Average 86 stars, based on 1 article reviews
primary antibodies against th - by Bioz Stars, 2026-09
86/100 stars

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Article Title: Continuous Low-Level Glial Cell Line-Derived Neurotrophic Factor Delivery Using Recombinant Adeno-Associated Viral Vectors Provides Neuroprotection and Induces Behavioral Recovery in a Primate Model of Parkinson's Disease
Article Snippet: Homogenates were diluted 1:1 with Laemmli solution buffer (Bio-Rad) containing 2.5% mercaptoethanol (Sigma) and were frozen. .. Four micrograms of protein from each sample were heated at 95–100°C for 5 min and then centrifuged before being loaded onto a 4 –20% SDS-PAGE gel (Criterion precast gel; Bio-Rad) and run for 2 h at 150 V. The gels were electroblotted onto a polyvinylidene difluoride membrane (Bio-Rad) and run for 2 h at 50 V. The membrane was probed with a primary antibody against TH (1:1000; Pel-Freez, Rogers, AR) overnight. .. The membrane was then incubated in anti-rabbit horseradish peroxide-linked secondary antibody (Amersham Biosciences) and then in hyperfilm ECL solution (Amersham Biosciences) for 1 min.

Membrane:

Article Title: Continuous Low-Level Glial Cell Line-Derived Neurotrophic Factor Delivery Using Recombinant Adeno-Associated Viral Vectors Provides Neuroprotection and Induces Behavioral Recovery in a Primate Model of Parkinson's Disease
Article Snippet: Homogenates were diluted 1:1 with Laemmli solution buffer (Bio-Rad) containing 2.5% mercaptoethanol (Sigma) and were frozen. .. Four micrograms of protein from each sample were heated at 95–100°C for 5 min and then centrifuged before being loaded onto a 4 –20% SDS-PAGE gel (Criterion precast gel; Bio-Rad) and run for 2 h at 150 V. The gels were electroblotted onto a polyvinylidene difluoride membrane (Bio-Rad) and run for 2 h at 50 V. The membrane was probed with a primary antibody against TH (1:1000; Pel-Freez, Rogers, AR) overnight. .. The membrane was then incubated in anti-rabbit horseradish peroxide-linked secondary antibody (Amersham Biosciences) and then in hyperfilm ECL solution (Amersham Biosciences) for 1 min.



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